2018, Cilt 10, Sayı 2, Sayfa(lar) 049-053
Comparison of DNA fragmentation rates in sperm samples prepared by microfluidic chip, swim-up and gradient methods
Tugce Murtaz1, Guler Ozturk2, Mehmet Cincik3, Belgin Selam4
1Institute of Health Sciences, Maltepe University, Istanbul Turkey
2Department of Physiology, Istanbul Medeniyet University School of Medicine, Istanbul Turkey
3Department of Histology and Embryology, Maltepe University School of Medicine, Istanbul Turkey
4Department of Obstetrics and Gynecology, Acibadem Mehmet Ali Aydinlar University, School of Medicine; Acibadem Altunizade Hospital, Unit of ART
Keywords: Sperm,DNA Fragmentation, TUNEL, Sperm Washing

Aim: Sperm preparation methods significantly effect the results of assisted reproduction techniques. Sperm washing removes cells potentially harmful to fertilization and obtains more motile and morphologically better sperm. Conventional sperm washing techniques, include centrifugal, non-centrifugal swim-up and centrifugal gradient methods. Sperm DNA fragmentation is one of the factors suggested for male infertility. Sperm washing methods may effect DNA fragmentation rate. The aim of the current study is to compare sperm DNA fragmentation rates between conventional sperm washing methods and relatively new microfluidic chip method.

Methods: Study group consisted of 14 volunteer men. Every sperm sample was allocated and washed separately in 3 categories as; gradient, non-centrifugal swim-up and finally microfluidic chip methods. Sperm DNA fragmentation rates in each category were compared by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) test.

Results:Ratio of TUNEL positive sperm with DNA fragmentation were 12%, 4% and 4,5% by gradient, non-centrifugal swim-up and microfluidic chip methods, respectively. Sperm DNA fragmentation ratio by gradient method statistically significantly increased compared to those with non-centrifugal swim-up and microfluidic chip methods (p<0.002). There was no statistical difference between non-centrifugal swim-up and microfluidic chip methods (p=0.583).

Conclusion: Current study determined increased sperm DNA fragmentation rate by gradient method compared to non-centrifugal swim-up and microfluidic chip methods. Centrifugation may increase oxidative stress, and thus lead to free radical production.